Through our exclusive agreement with the Gene Editing Institute of the Christiana Care Health System, we can provide you with state-of-the-art cell line engineering and production services. Our teams can disrupt or knock out genes, add or knock in DNA fragments, and/or create point mutations in genomic DNA.

Organizations that have used these services include the Wistar Institute, the Mayo Clinic, Dartmouth University, the University of Vermont, the University of North Dakota, and Thomas Jefferson University as well as major pharmaceutical companies.

Process & Outcomes

Our work begins once we have the NCBI Gene ID# for the gene that you want targeted for alteration. You specify your cell line of choice, along with your preferred culture conditions and transfection methods.

Unlike other commercial gene editing services, we take isoforms into account during CRISPR design. This ensures there are no functional protein variants even after frameshift mutations are created.

Given our methodical approach, we typically generate a series of various heterozygote and homozygous mutated cell lines. In some cases, these are extremely valuable because you can often observe gene dosage effects, avoid lethal effects, and more. All analyzed clones are provided as part of your order.

Timeline
The expansion of isolated modified cell lines in sufficient quantity for shipment is completely dependent on the speed at which the targeted cell lines divide.

Dedicated Project Management
We maintain close communication with you with weekly updates to inform you on the progress toward final clonal isolation and verification.

If cell morphology changes are observed after genomic modification, you’ll receive images as part of the update process. Once we analyze purified DNA samples from isolated clones, you’ll receive a vial of the modified cell line. For each final clone, we provide TIDE allelic analysis, describing the exact type of genetic modification that has occurred within the clonal isolate at each gene loci.

Pricing
While other commercial services require you to use limiting in-house cell lines or increase the price dramatically if you want to use a preferred line, we do all custom work for all cell lines at the same price.

Scale Up
Once we have engineered your cell line to your satisfaction, we can scale up large quantities of cells to provide ready-to-use cell banks, membrane preparations, proteins, and more.